The heartwarming picture, taken by the Princess of Wales, shows the former monarch in her final weeks at her Balmoral home in Scotland surrounded by her grandchildren and great-grandchildren.
A never-before-seen snap of the late Queen with some of her younger relatives has been released – and it is highly poignant.
Among them are Prince George, Princess Charlotte and Prince Louis along with Mike and Zara Tindall’s children as well as Lady Louise Windsor and James, the Earl of Wessex.
And eagle-eyed royal fans have been discovering touching hidden details within the image that you might not have noticed at first glance…
In the snap, the Queen sits alongside many of her great-grandchildren – although not all are present in the family snap.
Not featured are Prince Harry and Meghan Markle’s children Prince Archie and Princess Lilibet and neither is Princess Eugenie’s son August.
However, despite Princess Beatrice’s daughter Sienna not being in the picture, it appears she is featured in one of the framed photos standing proudly on the piano at the back of the room.
Fans believe the snap shows Sienna, who is 19 months, being held by her father Edoardo Mapelli Mozzi, in what appears to be a christening gown, meaning that snap was possibly taken after her baptism in April 2022.
One wrote on Twitter: “I saw Sienna Elizabeth Mapelli Mozzi’s picture frame with her father Edo Mapelli Mozzi.”
Another added: “We have yet to get any official photos or appearances of Sienna Mapelli Mozzi, but she can be seen with her father Edo in what looks like the throne room at St. James’s Palace after her Christening.
“Sienna was christened in the Chapel Royal at St. James’s Palace in April 2022.”
Meanwhile, there is another framed photo in a green tartan frame next to this, which appears to show the Queen holding a baby, with a man – possibly Prince Philip or King Charles – standing behind her. It is hard to tell who the baby is.
Louis’ hand-me-down from George
Standing behind the monarch in the touching snap is future King Prince George as well as his younger brother Prince Louis.
Louis, who turns five on Sunday, smiling sweetly wearing a smart blue striped shirt that comes complete with a grandad collar.
And it appears it could have been a garment passed down to him by his older brother, who appears to have worn it in his fourth birthday portraits from 2017.
The shirt that George wore in those photos features the exact same stripes, colour and distinctive collar.
The snap was taken at the late Queen’s beloved Balmoral home, where she spent her final hours.
When she would travel to Scotland, the late monarch would take her pet corgis with her – and the photo shows a home comfort for the dogs.
Underneath the table to the right of the image, next to Louis, is a novelty dog blanket that features a corgi design on them.
When the Queen died, she left behind her dogs Sandy and Muick, who are now cared for by Prince Andrew and his former wife Sarah Ferguson.
The youngest great-grandchild to feature in the snap is Zara and Mike Tindall’s son Lucas.
In a sweet move, he sits on his big sister Mia’s lap alongside the late monarch wearing an adorable pair of dungarees.
And if you look closely enough, you can see he is clutching what appears to be a piece of paper or cardboard.
Maybe it was something he had in his hand and simply refused to give up when it came to snapping the photo?
If so, it would echo big sister Mia, who in another snap with the former sovereign is seen holding her handbag after refusing to put it down for a photo.